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Microscopy Links
USEFUL MICROSCOPY LINKS

Brightfield Microscopy useful links
Below are some useful links on theory and applications in Microscopy
OLYMPUS Microscopy Resource Center. Kohler Illumination
OLYMPUS Microscopy Resource Center.
Introduction to Optical Microscopy, Digital Imaging, and Photomicrography
Microscopy and Digital Imaging
Royal Microscopical Society . International microscopical society
Fluorescence and Confocal Microscopy useful links
Below are some useful links on theory and applications in Microscopy
Electron Microscopy useful links
Below are some useful Electron Microscopy links;
Agar Scientific: Accessories for Microscopy
Oxford Instruments: INCA X-Sight Energy Dispersive Spectroscopy Detectors

Tissue processing and paraffin embedding of specimens
Paraffin embedding of specimens
It depends really on the size of the cartridge you want to use, small samples will fit a roughly measured 5mm dept, 27mm wide, 40mm long cartridge. Larger cartridges have a 10mm depth, with the other dimensions remaining the same. Usually 10-15 large cartridges can fit into a basket.
For paraffin embedding samples should be collected and stored in 4% Paraformaldhyde short term. For long term storage in 70% ethanol is recommended.
Parrafin embedding processing in the BSI, EM prep lab G.16
1. Dehydration and permeation in molten wax in the Histokinett http://www.leicabiosystems.com/specimen-preparation/tissue-processing/details/product/leica-tp1020/
(All automated, usually processed 10-15 samples on an overnight cycle 21hrs duration)
2. Wax blocking of samples on Tissue tec http://www.sakura-americas.com/products/tisstek-tec5.html
(Manual, processing is not difficult, need 2hrs max to process 10-15 samples)
3. Sectioning of samples on rotary microtome http://www.gmi-inc.com/leica-rm-2135-biocut-rotary-microtome.htm
(Manual, processing can be difficult especially in warmer weather, need a few days to section 10-15 blocks, depending on your method of sectioning)