LSR lasers and detector options
488nm Blue Laser – 2 detectors, 355nm UV Laser – 2 detectors, 405nm Violet Laser – 3 detectors, 561nm Yellow/Green Laser – 5 detectors, 640nm Red Laser – 3 detectors. This LSRII SORP can detect up to 17 parameters simultaneously, including scatter properties.
The unique 5-laser configuration of the LSRII offers the user the ability to measure up to 17 individual parameters simultaneously. The table below lists some of the fluorophores that can be used with the 5 different lasers.
| Laser | Dichroic | Filter | Filter Range (nm) | Detector | Name | Fluorophores |
|---|---|---|---|---|---|---|
| UV 355nm | 505LP | 525/50 | 500-550 | A | UV 1 | Indo-1 |
| 450/50 | 425-475 | B | UV 2 | Hoechst, Alexa Fluor 350, Marina Blue | ||
| Violet 405nm | 595LP | 610/20 | 600-620 | A | violet 1 | Qdot 605 |
| 505LP | 525/50 | 500-550 | B | violet 2 | Am Cyan, Pacific Orange, Alexa Fluor 430, Cascade Yellow | |
| 450/50 | 425-475 | C | violet 3 | Pacific Blue, Alexa Fluor 405, Cascade Blue | ||
| Blue 488 nm | 505LP | 525/50 | 500-550 | B | 488 2 | FITC, Alexa Fluor 488, GFP, CSFE |
| 675LP | 685/35 | 668-702 | A | 488 1 | PerCP, PE-Cy5 (TRI-COLOR), PE-Alexa Fluor 700, PerCP-Cy5.5, PE-Cy5.5, Propidium Iodide | |
| Yellow/Green 561 nm |
755LP | 780/60 | 750-810 | A | Y/G 1 | PE-Cy7, PE-Alexa Fluor 750 |
| 685LP | 710/50 | 685-735 | B | Y/G 2 | PE-Cy5.5, PE-Alexa Fluor 700, PE-Alexa Fluor 680 | |
| 635LP | 660/20 | 650-670 | C | Y/G 3 | PE-Alexa Fluor 647, PE-Cy5 | |
| 600LP | 610/20 | 600-620 | D | Y/G 4 | PE-TexasRed, PE-Alexa Fluor 610, Alexa Fluor 594 | |
| 582/15 | 574-589 | E | PE | PE | ||
| Red 640nm |
735LP | 780/60 | 750-810 | A | red 1 | APC-Cy7, APC-Alexa Fluor 750 |
| 685LP | 710/50 | 685-735 | B | red 2 | Alexa Fluor 700, APC-Cy5.5 | |
| 670/14 | 663-677 | C | red 3 | APC, Alexa Fluor 647 |