Experimental Modalities

• Angiography-vascular, coronaries, ventricle
• PCI, stent – invasive/non invasive assessment
• Hemodynamics
• Press volume loops, FFR, CFR
• 2-D , 3D angiography
• Regional wall, myocardial infarct size assessment Non invasive valve assessment
• Plaque area and volume assessment
• Coronary flow and ischemia analysis
• Biochemical and molecular tagging and tracking of molecules, tracers, cells, devices
• Potential for detection of unstable plaque
• Intravascular Ultrasound

Basic Science Investigative Resources

General

• Cell and Tissue Culture facilities (3 laminar flow hoods, 6 incubators)
• Full Protein, DNA and RNA analysis
• Western blotting, ELISA and HPLC
• RT-PCR including quantitative,
• Northern and Southern Blotting,
• RNAi of target genes
• Lentiviral vector design and production

Imaging

• Nikon TE-2000E Confocal Laser Microscopy
• Laser Intravital Microscopy
• Real-time cell analyses under flow
• Resin-embedding for Stented Vessel Sectioning

With collaborators

• Olympus Two-photon microscopy
• Olympus Spectral Scanning Confocal Microscopy

Flow Cytometry

• BD FACSCalibur 4-colour flow cytometer
• BD FACS Aria II 7-colour cell sorting

Small Animal Surgery

• Generation of bone marrow chimerics and transgenics
• Myocardial infarction; total occlusion or with reperfusion
• Angioplasty-mediated vascular injury