Platforms: Next Generation Sequencing

Overview

The APC Microbiome Institutes state of the art DNA sequencing technology allows researchers to:

• Accurately assess the composition or functional potential of the gut microbiota and microbiota of other environments (16S/ Shotgun metagenomic, RNAseq transcriptomics).
• Assess the microbiota impact on health and disease.
• Establish the impact of interventions (dietary, probiotic, prebiotic, antibiotic and/or chemotherapeutic) on these populations.
• Decipher the associated health promoting mechanisms of action of specific health promoting strains.
• Ensure the absence of virulence factors and assess the suitability of health-promoting strains for large scale industrial production.
• Facilitate the sequencing of host eukaryote DNA with RNAseq being particularly advantageous.

The services that the Sequencing Platform offers to users are:

• Project Design
• Standard and Custom Library Generation
• De Novo Sequencing of Genomes
• Re-Sequencing of Genomes
• Microbiome composition
• Functional genomics
• Transcriptome sequencing
• Data Assembly
• Data analyses