Investigation of the Effects of Catabolite Repression on Styrene Degradation in Pseudomanas putida CA-3

Investigation of the Effects of Catabolite Repression on Styrene Degradation in Pseudomanas putida CA-3

Funding Body (EPA, FP7, etc): EPA

Project type (PhD, post-doc, etc): PhD

Period (start to end): June 2008 - 2011

Researcher: Bill Ryan

Project Description

This project aims to investigate a critical, yet poorly understood, area of the microbial biodegradation of aromatic pollutants i.e. negative regulation of the ability of the strain to degrade pollutants in the presence of mixed carbon substrates referred to as catabolite repression.  By investigating and identifying the targets of this negative regulation in this strain, modification of DNA using recombinant technologies may provide a method of using the whole cell as a biocatalyst aimed at reducing toxic pollutant outputs from the petrochemical industry.  This studies outputs, although focusing on a single compound, Styrene, and one particular strain, P. putida CA-3, may also be relevant to many other biodegradation applications among similar Pseudomonads and may provide recombinant strains which could prove valuable to the bioremediation of contaminated landfill and in the treatment of stored waste aromatic compounds.

Environmental Research Institute

University College Cork, Lee Road, Cork

Top