Development and Validation of High-Throughput Screens to Identify Novel Inhibitors of Pathogenic Yeast, from Marine Sponge - Associated Microbes

Development and Validation of High-Throughput Screens to Identify Novel Inhibitors of Pathogenic Yeast, from Marine Sponge

Funding Body (EPA, FP7, etc) :Beaufort Biodiscovery Project

Project type (PhD, post-doc, etc) :PhD

Period (start to end) : October 2008 – October 2011

Researcher: Lekha Menon Margassery

Project Description

  1. To establish celluar targets of anti – fungal extracts of sponge associated bacteria using a Saccharomyces cerevisiae system.
  2. To develop a scaleable high – throughput assay to screen inhibitors of the calcium – activated phosphatase, Calcineurin, in yeast.
  3. To screen extracts of marine sponges – associated microbes and metagenomic libraries from such microbes to identify novel calcineurin inhibitors.
  4. To biologically chara cterise calcineurin inhibitors to determine specificity.
  5. To determine the chemical nature of novel inhibitory molecules.

Environmental Research Institute

University College Cork, Lee Road, Cork

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