Development and Validation of High-Throughput Screens to Identify Novel Inhibitors of Pathogenic Yeast, from Marine Sponge - Associated Microbes
Development and Validation of High-Throughput Screens to Identify Novel Inhibitors of Pathogenic Yeast, from Marine Sponge
Funding Body (EPA, FP7, etc) :Beaufort Biodiscovery Project
Project type (PhD, post-doc, etc) :PhD
Period (start to end) : October 2008 – October 2011
Researcher: Lekha Menon Margassery
- To establish celluar targets of anti – fungal extracts of sponge associated bacteria using a Saccharomyces cerevisiae system.
- To develop a scaleable high – throughput assay to screen inhibitors of the calcium – activated phosphatase, Calcineurin, in yeast.
- To screen extracts of marine sponges – associated microbes and metagenomic libraries from such microbes to identify novel calcineurin inhibitors.
- To biologically chara cterise calcineurin inhibitors to determine specificity.
- To determine the chemical nature of novel inhibitory molecules.