Pyrosequencing
Pyrosequencing
The Pyrosequencing platform provides sequencing of whole genomes, ultra-deep sequencing, metagenomics and RNA analysis
Tools/equipment/techniques:
- GS FLX Platform from Roche
- de novo sequencing & resequencing of whole genomes and target DNA regions, ultra-deep sequencing, metagenomics and RNA analysis
- 1,000,000 individual reads with improved Q20 read length of 400 bases per 10-hour instrument run
- Library preparation, capture, emulsification, amplification
- Bead recovery, enrichment, sequencing primer annealing
- Sequencing run
Platform Leaders: Paul Cotter, Fiona Crispie
Copyright
Alimentary Pharmabiotic Centre



