Utilisation of Microorganisms

in the field

The soil represents heterogenous habitat in which the environmental conditions can differ markedly from one microhabitat to the next. These different micro-habitats in the soil correspond to a large number of ecological niches, in which different microorganisms are present. Therefore, the microbial biodiversity of the soil ecosystem is very high. Soil microorganisms with certain traits of interest have been isolated from soil and are already used as inoculants (Table 1). However, the natural diversity of the microbial community of the soil has been little explored until recently and soil microorganisms represent a useful source for the isolation of new strains that could be exploited as microbial inoculants in agriculture or for industrial applications.

Selective cultivation media can be used to discriminate between microorganisms present in the rhizosphere.


Table 1

Bacteria currently used as agricultural inoculants in the field and new bacteria developed by genetic modification for improved performance.

Wild-type bacteria

Crop inoculated

Usage

Anabaena-Azolla
Azospirillum brasilense
and A. lipoferum

Rice
Cereals

Biofertilizer
Biofertilizer

Bradyrhizobium japonicum
Rhizobium spp.

Forage and grain legumes

Biofertilizer
Biofertilizer

Frankia spp.

Non-leguminous trees (Alnus)

Biofertilizer

Bacillus subtilis and B. thuringiensis
Psuedomonas fluorescens
Pseudomonas spp.

Different crops

Biopesticide
Biopesticide

Improved bacteria

Modified trait

Goal of modification

Agrobacterium radiobacter

Deletion of transfer genes

Safe use for biological control of crown gall

Clavibacter spp.

Addition of endotoxin from Bacillus thuringiensis

Biological control of insect damage to crops

Bradyrhizobium japonicum

Additional copies of nif gene

Increased nitrogen fixation

Pseudomonas syringae

Deletion of ice nucleation gene

Control of frost damage to crops

Rhizobium meliloti

Additional copies of nif and/or dct genes

Increased nitrogen fixation


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