BIOACTIVITY
We can determine the potential bioactivity of various compounds sourced from plants, the marine environment, dairy, meat, supplements and other sources.
The types of compounds we have tested include:
-
Phytochemicals:
- Carotenoids
- Flavonoids
- Plant sterols and stanols
- Herb extracts
- Softwood knot extracts
- Phytosterol oxidation products (POPs)
- Cholesterol oxidation products (COPs)
- N on-enriched and functional ingredient-enriched meat products
- Non-enriched and functional ingredient-enriched yoghurt
- Casein-derived hydrolysates
The different biomarkers we can determine include:
| Biomarker
|
Assays
|
| Toxicity | MTT, Neutral Red Uptake, Protein content, DNA synthesis |
| Cell membrane integrity |
FDA/EtBr, LDH release, Trypan Blue |
Antioxidant
|
DPPH, FRAP, Total Phenol Content/Folin-Ciocalteau Reducing Capacity, Ascorbic acid content Glutathione content, Catalase activity, Superoxide Dismutase activity, Reactive oxygen Species production |
| DNA integrity/damage |
Alkaline Single Cell Gel Electrophoresis (comet) |
| Apoptosis | Hoechst staining, DNA Fragmentation |
| Immune modulation |
Cell proliferation, Cytokine production, PKC Kinase activity |
