Research Study:

On-line monitoring of pollen and fungal spores in the field: a pilot study for MeteoSwiss,

Project Outline:

A 4.5-months long pilot field campaign was performed at the MeteoSwiss base in Payerne (March-July 2013) in order to compare the relative merits of traditional approaches to pollen/spore monitoring and counting with on-line techniques including the UCC WIBS (Waveband Integrated Bioaerosol Spectrometer).

Scientific Background:

The airborne monitoring of bioaerosols has become a research area attracting significant scientific interest over the last few years because of potential impacts on human health, plant-life and climate forcing. For example so-called Primary Aerosol Biological Particles (PBAP) such as pollen and fungal spores are known to induce allergic responses and thereby exacerbate reactions such as allergenic rhinitis (hay fever) and chronic obstructive pulmonary disease (COPD). These conditions are linked to exposures to certain species of biological particle.

Due to its novelty, methods based on the fluorescence of PBAP have been used only to a small extent for detecting and counting pollen by comparison to the impaction/light microscopy methodology. In fact, the only other studies (two) have been performed by us. Both campaigns were effective but carried out over only short (week-long) periods of time (in Killarney National Park and Munich). However they did act as a proof of principle that the WIBS on-line approach was able to give high correlation with results obtained by the traditional techniques. Hence the CRACLab team were invited to apply their expertise in a pilot study performed for MeteoSwiss at their monitoring station in Payerne during 2013.

The MeteoSwiss Campaign

The campaign focused mainly on the fine through to super coarse fraction of the ambient aerosol (ie particles with aerodynamic diameter between 1.5-22 µm). Increases in fluorescent particles were seen month on month from Spring to Summer and in addition a far greater fraction of the Summer total particles exhibited fluorescence due to their larger sizes. Diurnal trends for each of the months were constructed using both techniques. However more detailed information on the nature of particle fluorescence with regard to size and shape was also obtained.

The highest correlation for the results using the two techniques was in April, which coincided with the Birch pollination season. The reasons for this are that the size of Birch pollen fit exactly into the current WIBS sizing window. Very good correlations were also observed during the summer months, particularly when the largest amount of pollen (grass) were released. Additionally information on the release of fungal spores was obtained by the WIBS, a result that is extremely difficult and laborious to achieve with the traditional techniques because of their relatively small size and, often, hyaline (transluscent) natures. Important trends of pollen and spore release and detection as a function of various meteorological conditions (rainfall, relative humidity, wind direction/speed) were also determined in real-time.

Our initial conclusions were that currently (and mainly because of the lack of WIBS data-bases) that unambiguous identification of ambient bioparticles could not be attained. However the inherent capability of the collected real-time data was able to provide specific, high-risk days and times for analysts, using traditional impaction/light microscopy techniques, to focus on both for pollen and fungal spore identification/quantification. Hence more campaign experience and data-bases are required to help fulfill the obvious potential of WIBS. (A more extensive campaign of this type is to be performed in France during 2014).

Funding Details:

Funded by – MeteoSwiss, Switzerland
Funding period – 2013.
Funding cost - €25,000

Project Team:

Principal Investigators – Professor John Sodeau and Dr Bernard Clot
Post-doctoral Research Assistant – Dr David O’Connor

A project studying fine particles emitted from a metallurgical process (ferromanganese alloy manufacturer, Glencore Manganese France, in Dunkirk), to determine if and how properties of these particles (chemical composition, size, morphology) changed after being emitted. Our (the ATOFMS) role was to identify which ambient particles were produced by the ferromanganese plant, and to provide mixing state information for these particles. We also used the instrument to analyse samples from the plant's chimneys and of the ores used to make the alloy. Comparison between the ambient and sample mass spectra further confirmed sources and showed some differences in composition of particles at various stages of the alloy-making process and the effect ambient transport had

Website Link

Analysis of the Development and Occurence of Biological and Chemical Aerosols


Description

The main aims of this programme were to develop a complementary field monitoring and laboratory programme directed toward the analysis of natural (bioaerosol) components coincident with measurements of anthropogenic materials such as PM 10 and NO x for the Cork Harbour region. Novel instrumentation was used to investigate the Primary Biological Aerosol Particles (PBAP) in real-time and thereby provide key information on levels and identity of important allergens such as fungal spores and pollen. By this means an understanding of the processes by which particulate emissions, from both natural and anthropogenic sources are released to the Urban/Marine atmosphere in the Cork Harbour region will be developed. The EPA will thereby be provided with an assessment of the contribution of PBAP, PM 10 and NO x in both an inventory and source apportionment form.

The main target areas for study are:

- A thirty month continuous NO x (real-time) and PM 10 monitoring programme as a function of meteorological conditions, seasonality and composition within the Cork Harbour region.

- The proposed real-time measurements of PBAP will be the first in the world to use 2-D fluorescence technology developed in the last few years for the identification of airborne biological materials.

- A source apportionment model will be developed to increase our understanding of the way that anthropogenic pollutants interact with natural particulate-forming processes.

Project Team:

Staff: John Sodeau; Pat Whelan; Marcel Jansen
PDRA: Stig Hellebust and David Healy
PhD: EPA Scholarship, David O’Connor
M.Sc: Keith Linehan
PDRA: Crossover from ELIPSE, Ivan Kourtchev

Project Funding:

Period: 2008-2011
Cost: €736,381

Newsletter Download:

Biochea June 2009 Newsletter (1,419kB)

Report Download:

EPA CCRP Report No. 18 (4,936kB)

Environmental Linkages between In-Port Ship Emissions of Particulate Matter, their Chemical Analyses and Effects on Health

Research Study:

Composition and Sources of Particulate Air Pollution in a Port Environment, Cork, Ireland.

Project Outline:

A twenty-eight month air pollution measurement campaign was performed between 2007 and 2009 at two selected sites, Tivoli Docks (upper harbour) and Haulbowline Naval Base (mid-harbour) in Cork Harbour. Off-line chemical analyses of constituents of the particulate matter with particle diameters less 2.5 mm (PM2.5) were complemented by real-time and semi-continuous measurements of particulate-phase elemental carbon, organic carbon and sulfate. These quantitative measurements together with knowledge on the internal mixing state of particles, using single particle mass spectrometry, allowed statistical analyses to be performed in order to estimate both the relative and absolute contributions to ambient PM2.5 mass concentrations of the various sources of airborne pollution in Cork Harbour.

Scientific Background:

The collection of reliable information about source contributions to levels of key chemical species that are known to adversely impact on human health is crucial for devising effective air quality strategies. Also, from a public health perspective, it is very valuable to have detailed datasets regarding the characterisation of air pollution sources particularly when applied to urban, industrialised regions of key national importance.

PM can be emitted directly into the atmosphere from processes such as combustion (primary particles) or they can be produced in the atmosphere as a result of different chemical reactions (secondary particles).  The particles are generally classified by their size rather than composition or shape. Thus “coarse” particles have aerodynamic diameters larger than 2.5 mm and “fine” particles possess diameters lower than 2.5mm. The principal urban source of PM10 is direct from road traffic emissions and contains much dust, coal and oil fly ash. By contrast, analysis of PM2.5 shows it to contain many more secondary species such as sulfate/nitrate ions and organic compounds. Such size and chemical distinctions are important because inhaled particles with diameters greater than 2.5mm are removed in the head or upper respiratory tract, while those smaller than 2.5mm in diameter can reach the lungs thereby representing a potentially major risk for human health.

One relatively ignored source of PM is that derived from world-wide ship emissions. This is because seagoing ships are not subject to the stringent air quality legislation, which is applied to road transport. However various emission estimates (based on engine-type and total/type of fuel consumed) for vessels both in port and in transit have been performed recently on behalf of the EU. They demonstrate the fact that ships make significant contributions to our pollution inventories of NOx, SO2, organic compounds, PM and CO2. For example, the 2002 estimate of PM in-port emissions for Europe was 21 ktonne/annum (excluding fishing vessels) and is calculated to rise to 24-28 ktonne/annum by 2010. These figures are subject to an uncertainty of some ± 45% and clearly require some verification by field measurement programmes. Clearly, acidic sulfate aerosols can be formed from such emissions. Furthermore hazardous constituents from the combustion of marine fuels (“bunker fuels”), including arsenic, cadmium, chromium, lead, nickel, vanadium and polycyclic aromatic hydrocarbons (PAH) can also be released into the air.

There is no information available regarding size distributions of particles emitted from ship engine exhaust although it is expected that, in common with most large diesel engines, >80% of emitted particulate matter will be PM10 or less. Measurement data about the chemical composition is also sparse but some inferences can be drawn from the following facts:

  • It has been calculated that the PAH-emissions from one fairly large vessel entering port will correspond to those from 1200 heavy trucks.
  • Currently, 50,000 ppm maximum sulfur content for ship fuel operates and compares to a 10 ppm maximum for petrol in cars from 2007. The sulfur content of diesel fuel affects PM emissions because some of the sulfur in the fuel is converted to sulfate particles in the exhaust. The fraction converted to PM varies from one engine to another, but reducing sulfur decreases PM linearly in almost all engines. 
  • The average sulfur content of marine heavy fuel oil used in European waters is 27,000 ppm and it is estimated that by 2010 emissions from ships will equal three-quarters of the EU total for sulfur (and nearly two-thirds of that for nitrogen oxides).
    • Ships currently release about twice as much NOx/ton-km than trucks and it is estimated that 22% of the total NOx air-pollution deposition in Ireland comes from ship emissions.
    • SO2 and NOx can become converted into secondary sulfate and nitrate particles. Ship emissions are estimated to contribute between 20 and 30 % to the air concentrations of these particles in most coastal areas.
    • In-port PM emissions differ significantly from average emission values due to the occurrence of cold-start/warm-starts, the use of auxiliary engines and also to manoeuvring procedures because engine loads change rapidly.

Project Team:

Co-ordinator – Professor John Sodeau

Co-Principal Investigator – Dr John Wenger

Post-doctoral Research Assistant – Dr Stig Hellebust, Dr Arnaud Allanic, Dr Ivan Kourtchev

Research Assistant – Ian O’Connor, Jenny Bell

Funding Details:

Funded by - the Irish Environmental Protection Agency (EPA) - Science, Technology, Research & Innovation for the Environment (STRIVE) Programme.

Research theme - Environment and Human Health: a theme set up to improve knowledge to assist in the development and implementation of effective policy actions to reduce environmental impacts on human health.

Funding period – 2007-2011.
Funding cost - €360,000

Report Download:

EPA Strive Report Series No. 71

Environmental Research Institute - Transfer of Atmospheric & Science Knowledge.

Introduction

A full understanding of the impact of atmospheric pollution on human health and the ecology of the Earth System requires a scientific approach, which links laboratory experiments to field measurements to predictive models. The resultant environmental knowledge can then underpin accurate risk assessments, appropriate risk management strategies and effectively define “clean air” to society.

Epidemiological studies especially have emphasised the role played by particulate matter on health. Adverse outcomes include exacerbation of respiratory symptoms, reduced lung function, chronic bronchitis, cardiovascular diseases and mortality. However toxicological studies suggest that the health effect of particles do depend on their chemical composition, which is particle-size dependent.

The Eritask team includes postdoctoral competence in the following four areas in order to make linkages between the field collection of particles, analysis of their chemical composition, sources and their health effects:
  • Field measurement of particulate matter;
  • “Total” chemical analysis of the particulate matter;
  • Source apportionment modelling;
  • Biochemical toxicology of air particulate components.

Further information on

Particulate Matter (647kB)
Chemical Composition (412kB)
Environmental Effects (1,791kB)

• The Team

Prof. John Sodeau
Prof. James A. Heffron
Prof. John Wenger

Dr. Jose Manual Lopez Sebastian
Dr. Andrew Whittaker
Dr. Emma Pere Trepat
Dr. Virginia Silvari

Dr. David Healy
Mary Mahony
Dr. Ian O'Connor

• Funding

Marie Curie Actions
EPA
IRCSET

Links
EUROCHAMP Website
EUROCHAMP Database

Project Summary

The project integrates the most important environmental reaction chambers in Europe for studying atmospheric processes into a Europe-wide infrastructure. The consortium of partners provide their expertise and experience in atmospheric chemistry to researchers of different disciplines, policy and industry and offer an infrastructure that can be used by interested parties for solving a large variety of problems related to atmospheric science. The major goals of the project are:

- the initiation of an effective interdisciplinary collaboration between the community of atmospheric scientists and colleagues from other disciplines that are closely related to it. This will be achieved through the three networking activities of EUROCHAMP.

- the optimisation and further development of the infrastructures' performance. In order to meet these goals, two corresponding research activities are defined in the EUROCHAMP work programme, namely the development and refinement of analytical equipment and the development of chemical modelling techniques.

Besides the project partners, a number of selected associated user groups with a high grade of expertise in the different fields of interest provide their experience either as advisers to special topics or as potential users of the infrastructure.

Overall Description & Fundamental Objectives Of EuroChamp

The fundamental objective of the project is the integration of existing European research facilities to a grid of reaction chambers. These facilities were created by multinational initiatives to study the impact of atmospheric processes on regional photochemistry, global change, as well as cultural heritage and human health effects under most realistic conditions.

Although initial advances in the application of large chambers occurred in the United States, Europe now leads the world in the use of large, highly instrumented chambers for atmospheric model development and evaluation. Smaller chambers that were designed for specific purposes and are operated by experts in their fields excellently support such chambers. The integration of all these environmental chamber facilities within the framework of the EUROCHAMP infrastructure promotes retention of Europe's international position of excellence in this area and is unique in its kind worldwide.

The mobilisation of a large number of stakeholders dealing with environmental chamber techniques provides an infrastructure to the research community at a European level that offers a maximum support for a broad community of researchers from different disciplines. The EUROCHAMP project initiates a currently not existing structuring effect of atmospheric chemistry activities performed in European environmental chambers, since it offers the full availability of corresponding facilities for the whole European scientific community.

With respect to the project objectives mentioned above, three network activities and two joint research activities are formulated and cross-linked in the EUROCHAMP project.

Networking activities
The major objective of the networking activities within the EUROCAHMP project is the initiation of an effective interdisciplinary collaboration between the community of atmospheric scientists and colleagues from other disciplines that are closely related to it. This will be achieved through the three networking activities of EUROCHAMP.

Networking activity N1
The objective of networking activity N1 is the generation and application of standardised rules as a method of quality assurance for raw data analyses of the experiments in each facility. For this purpose a number of inter-comparison studies applying analytical devices in reference experiments will be carried in the different chambers, which provides an indirect measure of the infrastructures' excellence.

Networking activity N2
In order to make the results of experiments performed in the partners’ facilities most transparent and accessible to the scientific community, a standardised data protocol for chamber studies will be defined. This standardised form will be the basis for the central database of environmental chamber studies to be constructed within the project. This WWW-based database will be made accessible to the whole scientific community, leading to a most effective dissemination of the results.

Networking activity N3
Within networking activity N3 four larger international conferences / workshops on infrastructure-related topics will be organised. In order to reach a maximum of success, internationally established experts on the corresponding topics will be invited to join these conferences. The results will be published in suitable proceedings for dissemination to the scientific community.

Joint research activities

The major objective of the joint research activities within the EUROCHAMP project is the optimisation andfurther development of the infrastructures' performance. In order to meet these goals, two correspondingresearch activities are defined in the EUROCHAMP work programme, namely the development and refinement of analytical equipment and the development of chemical modelling techniques.

Joint research activity JRA1

The development of novel and the refinement of existing analytical devices of environmental chambers in order to successfully detect atmospheric trace species or to characterise aerosol particles is an essential task to be followed over the whole lifetime of the EUROCHAMP research facilities. The increasing demands for more comprehensive analytical techniques caused by the more and more complex scientific questions to be answered, requires a continuous improvement of the technical possibilities of a chamber.
Accordingly, the project includes a number of research activities focused on this topic:
- characterisation of oxygenated volatile organic compounds (OVOCs),
- radical measurements (OH, HO2, RO2),
- nitric acid measurements,
- characterisation of aerosols.

Besides the optimisation of existing devices (1st objective), a number of analytical devices will be completely new designed and introduced for the first time in an environmental chamber (2nd objective).

The highly specific equipment will be developed in a mobile form, so that such instruments may be transported to a chamber of choice and used in selected experiments independent of localisation. This philosophy strengthens the idea of a real grid of environmental chambers forming a powerful infrastructure. In addition, the instruments to be developed will be of great use for future field campaigns for which sophisticated, improved analytical instrumentation is urgently required.

Joint research activity JRA2

The field of chemical modelling is directly coupled to each type of environmental chamber studies. The analysis of chamber experiments without any model application is mostly not possible. Accordingly, model activities are urgently necessary and a permanent companion of each experimental task.

1. Since the quality of simulation studies strongly depends on the question how exactly the chemical behaviour of the chamber itself is characterised, sensitive parameters urgently required for simulations have to be determined for each facility of the infrastructure (1 st objective). The dissemination of each result from such studies serves for a better interpretability of environmental chamber studies as a whole.

2. The second objective for model applications is the test of complex chemical mechanisms used for multi-phase model applications related to chamber experiments. An established chemical code for interpretation of chamber studies that can be applied to all facilities increases the quality of the whole infrastructure and offers new possibilities for solving open questions of interest for the European researchers’ community.

3. Furthermore, chemical models to be developed can be applied in the EUROCHAMP network for the solution of specific problems in atmospheric chemistry, e.g. development and validation of degradation mechanisms of organic pollutants that are of paramount importance, the investigation of atmospheric reactivity as an overall property under various conditions or the influence of alternative fuels or solvents as well as bio fuels on tropospheric chemistry (3 rd objective).

In conclusion, this philosophy strengthens the idea of a real grid of environmental chambers as powerful tool for system analysis increasing the value of the whole chambers infrastructure for the European research community.

EUROCHAMP Partners

1. Bergische Universität Wuppertal, Wuppertal, Germany.
2. Forschungszentrum Jülich, Jülich, Germany.
3. Fundación Centro de Estudios Ambientales de Mediterráneo, Valencia, Spain.
4. Universität Bayreuth, Bayreuth, Germany.
5. University College Cork, Cork, Ireland.
6. Centre National de la Rechèrche Scientifique (CNRS-LCSR), Orleans, France.
7. Paul-Scherrer-Institute, Villigen, Switzerland.
8. University of Leeds, Leeds, United Kingdom.
9. SP Swedish National Testing and Research Institute, Borås, Sweden.
10. Karlshrue Institute of Technology, Karlshrue, Germany.
11. Université Paris 12, France.
12. Leibniz Institute for Tropospheric Research, Leipzip, Germany.
13. University of Copenhagen, Denmark.
14. University of Manchester, UK.

Research Study:

Studies of the Chemical Composition and Toxicity of Airborne Fine Particles in Cork's Mid-Harbour:
T
oxicological Analysis and Chemical Correlation for Fine PM in transit to Cobh (TACCo)

Project Outline:

Particulate matter (PM) was collected over a period of one year (April 2007 to April 2008) in Cork Harbour, Ireland. A high-volume cascade impactor and polyurethane foam collection substrates were used in the collection process. The inorganic constituents found in the fine fraction of PM obtained at the mid-harbour site (Haulbowline Naval Base) were characterised by inductively coupled plasma - optical emission spectroscopy (ICP-OES) and ion chromatography. The ability of the fine PM collected to induce toxic effects on biological systems was experimentally investigated. Oxidative stress, cytotoxicity and inflammatory responses were measured in an in vitro system using A549 human lung epithelial cells.

Funding Details:

Funded by - the Irish Environmental Protection Agency (EPA) - Science, Technology, Research & Innovation for the Environment (STRIVE) Programme.

Research theme - Environment and Human Health: a theme set up to improve knowledge to assist in the development and implementation of effective policy actions to reduce environmental impacts on human health.

Funding period – 2009-2010.

Funding cost - €80,000

Additional Funding Bodies – IRCSET (PhD Scholarship), Cork City Council.

Scientific Background:

The World Health Organization has estimated that exposure to fine particulate matter (PM) in outdoor air leads to about 100,000 deaths annually in Europe and that the effect of PM on life expectancy may be of the order of one to two years. Causal linkage between atmospheric particles and human health has been proposed to depend on many factors including size, physical state and chemical composition. However exact interpretation of particulate behaviour in this regard is complex because PM sources vary widely and few studies have related their physical and chemical nature to toxicological testing. What is known, from many previous monitoring studies, is that airborne particles may be solid or liquid in nature and can possess diameters that vary between 0.002 and 100μm. However their shapes are rarely spherical and may be rod-like in nature or totally irregular. They are comprised of trace metals and other inorganic materials, elemental carbon, a variety of organic compounds and also water in varying ratios. Furthermore mortality rates, particularly in urban areas, have been statistically linked to levels of atmospheric particulates when acute lower respiratory symptoms have been observed.

Current interest in the health impacts related to chemical composition of the two types of PM are based on two main tenets: (1) the possibility that they contain surface-adsorbed carcinogens such as polyaromatic hydrocarbons (PAH), which can be released into the body; (2) the promotion of oxygen radical reactions by increasing the bio-availability of certain metals in particulate acidic environments. However before real progress is made in confirming the importance of these ideas many more source types of PM must be chemically analysed and physically characterized before being tested for bio-reactivity.

As mentioned above, the acidity of the emitted PM is important because it can control the bio-availability of many trace metals. This phenomenon is crucial because it has been shown in animal studies that the acute toxicity associated with certain atmospheric dusts is most likely the result of the levels of soluble zinc, vanadium, nickel and sulfate ions present. In other words toxicological effects, such as pulmonary hyper-reactivity and lung damage, caused by the deposition of particulate matter in the airways may depend on their aqueous leachable chemical constituents. All of the above materials have been observed in urban field measurements of particulate matter throughout the world because diesel contains many trace elements resulting from the crude oil sources and catalytic processes, which transform diesel distillate to diesel fuel.

Project Team:

Co-ordinator – Professor John Sodeau

Post-doctoral Research Assistant – Dr John Menton, Dr Siobhan Cashman

Research Assistant – Ian O’Connor

Report Download:

EPA Strive Report Series No. 85

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